Phospho-JUN Antibody C-J 4C4/1 / Phospho-c-Jun T91/T93 Antibody [clone C-J 4C4/1] (V7427)

Phospho-JUN Antibody C-J 4C4/1 Shows Nuclear Staining in Cervical Tumor IHC. Immunohistochemistry testing of FFPE human cervical tumor tissue with Phospho-JUN Antibody clone C-J 4C4/1. HIER was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. Strong brown staining is localized predominantly to tumor cell nuclei across the field, with comparatively little cytoplasmic signal. The widespread nuclear labeling demonstrates detection of phosphorylated JUN in cervical tumor tissue using this Phospho-c-Jun T91/T93 Antibody.

Phospho-JUN Antibody C-J 4C4/1 in Human Cervical Tumor IHC. Immunohistochemistry testing of FFPE human cervical tumor tissue with Phospho-JUN Antibody clone C-J 4C4/1. HIER was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. The image shows strong predominantly nuclear brown staining throughout the squamous tumor cell population, with comparatively little staining in the surrounding stromal tissue. The nuclear localization is consistent with detection of phosphorylated JUN and demonstrates tissue staining with this Phospho-c-Jun T91/T93 Antibody.

Purified Phospho-JUN Antibody C-J 4C4/1 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Phospho-JUN Antibody clone C-J 4C4/1 under non-reducing (NR) and reducing (R) conditions with a 2 ug antibody load. The non-reduced preparation shows the predominant intact antibody at approximately 150 kDa. Following reduction, the preparation resolves into immunoglobulin heavy and light chains at approximately 50 kDa and 25 kDa, respectively. The expected electrophoretic profile provides a quality-control assessment of the integrity and purity of this Phospho-c-Jun T91/T93 Antibody preparation.