Mammaglobin Antibody - Protein Microarray Validated / SCGB2A2 Antibody [clone MGB/2000] (V3862)

Mammaglobin Antibody - Protein Microarray Validated - immunohistochemistry of human breast carcinoma. FFPE human breast carcinoma tissue was stained with Mammaglobin Antibody - Protein Microarray Validated (clone MGB/2000) to detect Mammaglobin A / SCGB2A2. HRP-DAB brown chromogenic signal highlights cytoplasmic staining in carcinoma epithelial cells within glandular tumor structures, consistent with the secretory localization of this breast-associated protein. Surrounding stromal cells show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM citrate buffer (pH6) for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.

IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human SK-BR-3 cell lysate (mammary gland tumor cell line) with Mammaglobin antibody (clone MGB/2000). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

Mammaglobin Antibody - Protein Microarray Validated clone MGB/2000 - microarray specificity validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins was performed using Mammaglobin Antibody - Protein Microarray Validated (clone MGB/2000). The strongest binding signal corresponds to SCGB2A2 (Mammaglobin A), which ranks first on the array, demonstrating high specificity of the MGB/2000 mouse monoclonal antibody for its intended target. Z- and S-scores quantify antibody binding strength and target specificity. The Z-score represents the signal intensity produced when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a protein on the array and is expressed as standard deviations above the mean signal of the array. When proteins are ordered by descending Z-score, the S-score represents the difference between adjacent Z-scores and therefore reflects the relative specificity of the antibody for its target protein.

SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone MGB/2000) as confirmation of integrity and purity.

Mammaglobin Antibody - Protein Microarray Validated - immunohistochemistry of human breast adenocarcinoma. FFPE human breast adenocarcinoma tissue was stained with Mammaglobin Antibody - Protein Microarray Validated (clone MGB/2000) to detect Mammaglobin A / SCGB2A2. HRP-DAB brown chromogenic signal highlights cytoplasmic staining in carcinoma epithelial cells distributed throughout the tumor tissue, consistent with expression of this breast-associated secretory protein in malignant mammary epithelial cells. Surrounding stromal elements show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.