FXN Antibody / Mitochondrial Iron Metabolism Antibody [clone FXN/2124] (V3992)

FXN Antibody Pancreatic Acinar Cell IHC. Immunohistochemistry staining of FFPE human pancreas using FXN antibody demonstrates diffuse granular cytoplasmic HRP-DAB brown staining within metabolically active pancreatic acinar cell populations, consistent with mitochondrial localization of Frataxin / FXN and its role in iron-sulfur cluster biogenesis and oxidative metabolic regulation. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

FXN Antibody Mitochondrial Metabolism IHC. Immunohistochemistry staining of FFPE human pancreas tissue using FXN antibody reveals strong cytoplasmic HRP-DAB brown staining in clustered epithelial cell populations with a punctate mitochondrial-associated distribution pattern characteristic of Frataxin / FXN expression in high energy-demand tissue compartments. The observed staining profile supports the established role of FXN in mitochondrial iron homeostasis and oxidative phosphorylation-associated pathways. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

FXN Antibody Dual Cell Line WB. Western blot analysis of human Panc-2 and HepG2 cell lysates using monoclonal clone FXN/2124 demonstrates a prominent band near 14-15 kDa, consistent with the mature processed form of Frataxin / FXN following mitochondrial import-associated cleavage. The observed signal in metabolically active pancreatic and hepatic-derived cell lines supports expression of this mitochondrial iron metabolism protein involved in iron-sulfur cluster biogenesis and oxidative phosphorylation regulation.

FXN Antibody Protein Microarray Specificity Analysis. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using monoclonal clone FXN/2124 identified Frataxin / FXN as the dominant target with a Z-score of 125.91 and an S-score of 104.11, supporting exceptionally strong target specificity relative to unrelated proteins present on the array. The markedly elevated S-score demonstrates highly preferential recognition of FXN with minimal off-target reactivity. The Z-score represents signal intensity generated by antibody binding relative to the mean signal across the array, while the S-score reflects the difference in signal strength between successive ranked proteins and serves as a measure of relative target specificity.

SDS-PAGE analysis of purified, BSA-free FXN antibody (clone FXN/2124) as confirmation of integrity and purity.