HML-1 Antibody ITGAE/2474 / Mucosal Lymphocyte 1 Antigen Antibody [clone ITGAE/2474] (V3837)

HML-1 Antibody ITGAE/2474 Staining in Human Tonsil by IHC. Immunohistochemistry of FFPE human tonsil tissue using HML-1 Antibody (clone ITGAE/2474) shows strong brown staining in a distinct population of scattered lymphoid cells against a densely hematoxylin-counterstained background. Positive cells vary in size and are distributed in clusters and individually throughout the field. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 1 mM EDTA buffer for 10-20 min followed by cooling at RT for 20 min. The selective cellular pattern demonstrates detection of CD103-positive lymphocytes using this Mucosal Lymphocyte 1 Antigen Antibody.

HML-1 Antibody ITGAE/2474 Staining in Human Colon by IHC. Immunohistochemistry of FFPE human colon tissue using HML-1 Antibody (clone ITGAE/2474) shows distinct brown staining in numerous cells associated with the colonic mucosa. Positive cells are concentrated within and around the glandular structures, while the surrounding nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 1 mM EDTA buffer for 10-20 min followed by cooling at RT for 20 min. This mucosal staining demonstrates detection of CD103-expressing cells using this Mucosal Lymphocyte 1 Antigen Antibody.

HML-1 Antibody ITGAE/2474 Staining in Human Small Intestine by IHC. Immunohistochemistry of FFPE human small intestine tissue using HML-1 Antibody (clone ITGAE/2474) shows localized brown staining in cells distributed within the intestinal mucosa and villous structures. Positive cells are interspersed among predominantly hematoxylin-counterstained epithelial and stromal cells, producing a heterogeneous cellular pattern. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 1 mM EDTA buffer for 10-20 min followed by cooling at RT for 20 min. The observed pattern supports detection of mucosal CD103-positive cells using this Mucosal Lymphocyte 1 Antigen Antibody.

HML-1 Antibody ITGAE/2474 Protein Microarray Validated. HuProt protein microarray analysis containing more than 19,000 full-length human proteins demonstrates highly selective recognition of CD103 by HML-1 Antibody (clone ITGAE/2474). CD103 ranks first with a Z-score of 108.07, compared with 8.3 for the next-ranked protein, PCDH17, producing an S-score of 99.77. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next-ranked protein. The exceptionally large separation supports high target specificity for this Mucosal Lymphocyte 1 Antigen Antibody.

HML-1 Antibody ITGAE/2474 Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free HML-1 Antibody (clone ITGAE/2474) with 2 ug loaded per lane shows the expected antibody migration profiles under non-reducing (NR) and reducing (R) conditions. The non-reduced antibody migrates predominantly near 150 kDa, while reduction produces the characteristic immunoglobulin heavy and light chain bands at approximately 50 kDa and 25 kDa. Limited additional staining is visible, supporting the integrity and purity of this Mucosal Lymphocyte 1 Antigen Antibody preparation.