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TTC36 Antibody / Tetratricopeptide repeat protein 36

Catalog No : FY13091
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse
Format : Lyophilized
Immunofluorescent staining of TTC36 using anti-TTC36 antibody (green) and anti-Beta Tubulin antibody (red). TTC36 was detected in immunocytochemical section of cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TTC36 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. TTC36 antibody (green) shows nuclear-enriched staining with nucleolar sparing, consistent with reported nuclear localization. Beta-tubulin antibody (red) marks the cytoplasmic microtubule network surrounding the nucleus.
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