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banner Search results for TBC1D1

TBC1D1 Antibody / TBC1 domain family member 1

Catalog No : FY12441
Applications : WB, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of TBC1D1 using anti-TBC1D1 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBC1D1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. TBC1D1 (~130-135 kDa predicted) was detected primarily at ~150-160 kDa, frequently as a doublet, consistent with phosphorylation-dependent mobility shifts reported for TBC1D1 in response to insulin, AICAR, and contraction.
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TBC1D13 Antibody

Catalog No : F54624
Applications : IF, IHC-P, WB
Reactivity : Human, Mouse
Format : Purified
Western blot testing of human CCRF-CEM cell lysate with TBC1D13 antibody. Predicted molecular weight ~47 kDa.
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