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SURF6 Antibody / Surfeit locus protein 6

Catalog No : FY13209
Applications : WB, ICC, IF, ELISA
Reactivity : Human
Format : Lyophilized
Immunofluorescent staining of SURF6 using anti-SURF6 antibody (red) and anti-Beta Tubulin antibody (green). SURF6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SURF6 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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