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STK17A Antibody / Serine/threonine-protein kinase 17A

Catalog No : FY12534
Applications : WB, IP, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Immunoprecipitating STK17A in U251 whole cell lysate. Western blot analysis of STK17A using anti-STK17A antibody. Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-STK17A antibody in U251 whole cell lysate, Lane 3: anti-STK17A antibody (2ug) + U251 whole cell lysate (500ug). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STK17A antibody at a dilution of 0.5 ug/ml and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate. STK17A (~47 kDa predicted) was detected as a major band at ~53 kDa, consistent with phosphorylation-dependent migration shift and the acidic C-terminal domain known to cause slower SDS-PAGE mobility.
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