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SUPT6H Antibody / SPT6 / Transcription elongation factor SPT6

Catalog No : FY13391
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Monkey, Mouse, Rat
Format : Lyophilized
Western blot analysis of SUPT6H using anti-SPT6 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: monkey COS-7 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUPT6H antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. A strong band was detected at approximately 250 kDa, which is higher than the predicted 199 kDa and consistent with the known slow-migrating behavior of SUPT6H caused by its acidic, low-complexity domains and extensive phosphorylation.
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