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  • Tel: 858.663.9055
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SPIDR Antibody / Scaffold protein involved in DNA repair

Catalog No : FY12783
Applications : ELISA, IF, ICC, WB, FACS
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of SPIDR using anti-SPIDR antibody and anti-Beta Tubulin antibody. SPIDR was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SPIDR antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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