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banner Search results for SPAG16

SPAG16 Antibody / Sperm-associated antigen 16

Catalog No : FY12458
Applications : WB, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of SH-SY5Y cells using anti-SPAG16 antibody. Overlay histogram showing SH-SY5Y cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAG16 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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SPAG16 Antibody

Catalog No : R36491
Applications : IHC-P, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Mouse, Dog, Cow
Format : Antigen affinity purified
IHC testing of FFPE human testis tissue with SPAG16 antibody at 5ug/ml. HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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