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SIGLEC15 Antibody / Sialic acid-binding Ig-like lectin 15

Catalog No : FY12474
Applications : WB, ICC/IF, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Immunofluorescent staining of SIGLEC15 using anti-SIGLEC15 antibody (green) and anti-Beta Tubulin antibody (red). SIGLEC15 was detected in an immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SIGLEC15 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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