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POLR1A Antibody / DNA-directed RNA polymerase I subunit RPA1

Catalog No : FY12855
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of POLR1A using anti-POLR1A antibody. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1A antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for POLR1A at approximately 195 kDa. The expected molecular weight of POLR1A is ~195 kDa.
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