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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
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PLS1 Antibody / Plastin-1

Catalog No : FY12925
Applications : WB, IHC, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of PLS1 using anti-PLS1 antibody. Lane 1: human whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PLS1 at approximately 70 kDa. The expected molecular weight of PLS1 is at 70 kDa.
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