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banner Search results for PDE4B

PDE4B Antibody

Catalog No : R33479
Applications : WB, IHC-P, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Mouse, Rat
Format : Antigen affinity purified
Western blot testing of mouse brain lysate with PDE4B antibody at 0.5ug/ml. Predicted molecular weight: ~83 kDa, routinely observed at 83~98 kDa.
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PDE4B Antibody

Catalog No : R34331
Applications : WB, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Mouse
Format : Antigen affinity purified
Western blot testing of human uterus lysate with PDE4B antibody at 2ug/ml. PDE4B (~83 kDa predicted) was detected at 95-97 kDa, consistent with expression of the longer PDE4B5 isoform and phosphorylation-dependent mobility shift characteristic of PDE4 family enzymes.
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PDE4B Antibody / Phosphodiesterase 4B

Catalog No : FY12523
Applications : WB, ELISA
Reactivity : Human, Mouse
Format : Lyophilized
Western blot analysis of PDE4B using anti-PDE4B antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4B antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. PDE4B (~83 kDa predicted) was detected at 95-97 kDa, consistent with expression of the longer PDE4B5 isoform and phosphorylation-dependent mobility shift characteristic of PDE4 family enzymes.
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