• Tel: 858.663.9055
  • separatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for PCGF6

PCGF6 Antibody / Polycomb group RING finger protein 6

Catalog No : FY12771
Applications : WB, FACS
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of U251 cells using anti-PCGF6 antibody. Overlay histogram showing U251 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCGF6 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
›
img img