• Tel: 858.663.9055
  • separatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for PCDHA11

PCDHA11 Antibody / Protocadherin alpha 11

Catalog No : FY12263
Applications : WB, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of PCDHA11 using anti-PCDHA11 antibody. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHA11 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for PCDHA11 at approximately 150-170 kDa. The expected band size for PCDHA11 is at 103 and 88 kDa but may be observed at higher molecular weights due to glycosylation.
img