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OGFOD3 Antibody / 2-Oxoglutarate and iron-dependent oxygenase domain-containing protein 3

Catalog No : FY12709
Applications : WB, IHC, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of OGFOD3 using anti-OGFOD3 antibody (green) and anti-Beta Tubulin antibody (red). OGFOD3 was detected in immunocytochemical section of cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-OGFOD3 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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