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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for NOP2

NSUN1 Antibody / NOP2 / Nucleolar protein 2

Catalog No : RQ8312
Applications : WB, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolar protein 2 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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NSUN1 Antibody / NOP2 / Nucleolar protein 2

Catalog No : F53988
Applications : WB
Reactivity : Human
Pred. Reactivity :Mouse
Format : Antigen affinity purified
Western blot testing of human 1) K562 and 2) U-2OS cell lysate with NOP2 antibody at 1:2000. Predicted molecular weight: 89 kDa but routinely observed at 100~120 kDa.
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NSUN4 Antibody / NOP2/Sun RNA methyltransferase 4

Catalog No : FY12068
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of NSUN4 using anti-NSUN4 antibody. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected band size for NSUN4 is at 43 kDa.
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NSUN6 Antibody / NOP2/Sun RNA methyltransferase family member 6

Catalog No : FY12291
Applications : WB, IHC, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of NSUN6 using anti-NSUN6 antibody. NSUN6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NSUN6 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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NSUN7 Antibody / NOP2/Sun RNA methyltransferase family member 7

Catalog No : FY12928
Applications : WB, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of PC-3 cells using anti-NSUN7 antibody. Overlay histogram showing PC-3 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSUN7 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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