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NOL9 Antibody / Nucleolar protein 9

Catalog No : FY12432
Applications : WB, ICC/IF, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Immunofluorescent staining of NOL9 using anti-NOL9 antibody (green) and anti-Beta Tubulin antibody (red). NOL9 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-NOL9 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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