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NDUFB11 Antibody / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11

Catalog No : FY12865
Applications : WB, IHC, ICC/IF
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of NDUFB11 using anti-NDUFB11 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB11 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of NDUFB11 is ~17 kDa.
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