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LYRM4 Antibody / LYR motif-containing protein 4

Catalog No : FY12618
Applications : WB, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of LYRM4 using anti-LYRM4 antibody. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRM4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot probed with anti-LYRM4 shows a major band at ~11 kDa, lower than the predicted ~15 kDa, consistent with the mature mitochondrial form of LYRM4 lacking its cleaved N-terminal targeting sequence.
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