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LSM11 Antibody / U7 snRNA-associated Sm-like protein LSm11

Catalog No : FY13034
Applications : WB, ELISA
Reactivity : Human, Rat
Format : Lyophilized
Western blot analysis of LSM11 using anti-LSM11 antibody. Lane 1: rat testis tissue lysates, Lane 2: rat skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM11 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A distinct band is observed at approximately 68 kDa, higher than the predicted molecular weight of 40 kDa. This upward shift is consistent with reports that LSM11 forms detergent-resistant complexes with FLASH and LSM10 and can undergo phosphorylation-dependent mobility shifts. The ~68 kDa band likely represents the complexed or post-translationally modified form of LSM11 detected under standard denaturing conditions.
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