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KDM8 Antibody / Lysine-specific demethylase 8

Catalog No : FY12823
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of KDM8 using anti-KDM8 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM8 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at ~60 kDa in HeLa, consistent with full-length KDM8 migrating above its predicted ~47 kDa due to anomalous mobility and post-translational modification.
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