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GALNT15 Antibody / Polypeptide N-acetylgalactosaminyltransferase 15

Catalog No : FY12022
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of GALNT15 using anti-GALNT15 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT15 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. A specific band was detected for GALNT15 at approximately 73 kDa. The expected band size for GALNT15 is at 73 kDa.
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