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FGD5 Antibody / FYVE, RhoGEF and PH domain-containing protein 5

Catalog No : FY12385
Applications : WB, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of 293T cells using anti-FGD5 antibody. Overlay histogram showing 293T cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGD5 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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