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ESD Antibody / Esterase D

Catalog No : FY12168
Applications : WB, ICC/IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of ESD using anti-ESD antibody (green) and anti-Beta Tubulin antibody (red). ESD was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-ESD antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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