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DDIT4 Antibody / REDD-1

Catalog No : F54848
Applications : WB, IHC-P
Reactivity : Human
Format : Purified
REDD-1 Antibody Human Adenocarcinoma IHC. IHC staining of FFPE human adenocarcinoma tissue with REDD-1 antibody demonstrated cytoplasmic staining in malignant epithelial cells, consistent with the expression pattern of REDD-1 (DDIT4), a stress-responsive regulator of mTOR signaling. REDD-1 is induced by hypoxia, oxidative stress, DNA damage, and metabolic challenges, where it functions to suppress mTOR activity and promote cellular adaptation to adverse conditions. The staining observed within tumor cells is consistent with activation of stress-response pathways commonly associated with the tumor microenvironment. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes and allowing them to cool prior to staining. These results support the utility of REDD-1 Antibody for studies of hypoxia signaling, mTOR pathway regulation, cellular stress responses, and cancer biology.
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DDIT4 Antibody / REDD-1 / Zebrafish

Catalog No : RZ1462
Applications : WB
Reactivity : Zebrafish
Format : Antigen affinity purified
Zebrafish DDIT4 Antibody WB. Western blot analysis of DDIT4 using anti-DDIT4 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT4 antigen antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus WB. Western blotting Substrate with Tanon 5200 system. A specific band was detected for DDIT4 at approximately 24 kDa. The expected band size for DDIT4 is at 24 kDa.
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