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Carf Antibody / Collaborator of ARF / Cdkn2aip

Catalog No : FY13379
Applications : WB, FACS, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of mouse NIH/3T3 cells using anti-Carf antibody. Overlay histogram showing NIH/3T3 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carf antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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