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CYP4F2 Antibody / Cytochrome P450 4F2

Catalog No : FY12148
Applications : WB
Reactivity : Human
Format : Lyophilized
Western blot analysis of CYP4F2 using anti-CYP4F2 antibody. Lane 1: human whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP4F2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of CYP4F2 is ~60 kDa (preprocessed form) and ~50 kDa (cleaved mature form).
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