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CLOCK Antibody / CLOCKA / Zebrafish

Catalog No : RZ1338
Applications : WB
Reactivity : Zebrafish
Format : Antigen affinity purified
Zebrafish CLOCK / CLOCKA Antibody Circadian Rhythm WB. Western blot analysis of whole female zebrafish tissue lysate using Zebrafish CLOCK Antibody demonstrates a prominent immunoreactive band at approximately 100 kDa, consistent with the predicted molecular weight of Circadian Locomotor Output Cycles Kaput A (CLOCKA). CLOCKA is a core circadian transcription factor that regulates rhythmic gene expression and functions within the molecular clock machinery controlling biological timing, metabolism, development, and behavioral rhythms. Additional weak higher molecular weight immunoreactive bands are observed and may represent modified forms of CLOCKA associated with transcriptional regulation and circadian signaling. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate.
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