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banner Search results for ATP6V1B2

Atp6v1b2 Antibody / Vacuolar Proton Pump Subunit B / Zebrafish

Catalog No : RZ1130
Applications : WB, IHC-P
Reactivity : Zebrafish
Format : Antigen affinity purified
IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp6v1b2 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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ATP6V1B2 Antibody / ATPase H+ transporting V1 subunit B2

Catalog No : FY12679
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of ATP6V1B2 using anti-ATP6V1B2 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1B2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for ATP6V1B2 at approximately 57 kDa. The expected molecular weight of ATP6V1B2 is ~57 kDa.
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