NSJ Bioreagents

Sarcomeric Alpha Actinin / ACTN2 is a structural protein that binds to actin filaments in the sarcomeres of muscle fibers. It acts as a crosslinker, providing stability and support to the sarcomere structure during muscle contraction. Without Sarcomeric Alpha Actinin, muscles would not be able to generate the force needed for movement. Sarcomeric Alpha Actinin plays a critical role in muscle function by anchoring actin filaments to the Z-discs of the sarcomere. This allows for the transmission of force generated during muscle contraction, leading to movement. Additionally, Sarcomeric Alpha Actinin helps to maintain the proper alignment of actin filaments, ensuring efficient muscle contraction. Studies have shown a correlation between Sarcomeric Alpha Actinin levels and muscle hypertrophy. By targeting Sarcomeric Alpha Actinin through exercise and training, athletes may be able to enhance muscle growth and improve overall athletic performance. Ensuring adequate levels of Sarcomeric Alpha Actinin is essential for maintaining muscle health and preventing injuries. Individuals with deficiencies in Sarcomeric Alpha Actinin may be more susceptible to muscle strain, tears, and other injuries. By incorporating exercises that target and strengthen Sarcomeric Alpha Actinin, individuals can improve muscle stability and reduce the risk of injury.

The Sarcomeric Alpha Actinin antibody can be used in various research techniques such as western blot, immunohistochemistry, immunofluorescence, and flow cytometry to discover more about this protein.

Western blotting, a widely used technique in molecular biology, relies heavily on the specificity and sensitivity of antibodies to detect and quantify proteins of interest. The Sarcomeric Alpha Actinin antibody has been proven to be a trusty companion in this technique, allowing researchers to unravel the presence and abundance of this crucial protein in various biological samples.

Immunohistochemistry enables researchers to visualize the distribution and localization of proteins within tissues. The ACTN2 antibody plays a key role in identifying the presence of this vital protein in tissue sections, aiding in the understanding of its role in muscle function and development.

Immunofluorescence, a powerful tool in cell biology, allows researchers to visualize proteins within cells with high spatial resolution. With the aid of a Sarcomeric Alpha Actinin antibody, researchers can accurately map the distribution of this protein within muscle cells, shedding light on its structural and functional significance.

Flow cytometry is a high-throughput technique for analyzing and sorting cells based on specific protein markers, relies on the specificity and reliability of antibodies for accurate results. The Sarcomeric Alpha Actinin antibody enabling researchers to identify and quantify the expression of this protein in different cell populations.

The Sarcomeric Alpha Actinin antibody proves to be a versatile and indispensable tool in various research techniques, ranging from western blotting to flow cytometry. Its specificity, sensitivity, and reliability make it a must-have for researchers exploring the intricate world of muscle biology.

Researchers studying cardiac muscle organization, sarcomere assembly pathways, and contractile cytoskeletal regulation may also be interested in our Cardiovascular Antibodies landing page featuring endothelial markers, signaling mediators, and cardiovascular disease related targets.

Browse the complete collection of research antibodies on our Antibodies landing page.

<p><strong data-start="0" data-end="58" data-is-only-node="">Sarcomeric Alpha Actinin Antibody Skeletal Muscle IHC.</strong> Immunohistochemistry analysis of FFPE human skeletal muscle tissue using recombinant Sarcomeric Alpha Actinin Antibody clone ACTN2/7041R demonstrates strong HRP-DAB brown staining throughout striated muscle fibers. The staining pattern highlights the organized contractile architecture of skeletal muscle and is consistent with the established localization of Alpha Actinin 2 (ACTN2), a major Z-disc-associated protein that cross-links actin filaments and supports sarcomere organization. This image illustrates the highly ordered structure of the muscle contractile apparatus and supports studies of muscle biology, sarcomere assembly, and cardiovascular research. The inset image represents a negative control in which PBS was used in place of the primary antibody and shows absence of specific staining. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.</p>

Sarcomeric Alpha Actinin Antibody Skeletal Muscle IHC. Immunohistochemistry analysis of FFPE human skeletal muscle tissue using recombinant Sarcomeric Alpha Actinin Antibody clone ACTN2/7041R demonstrates strong HRP-DAB brown staining throughout striated muscle fibers. The staining pattern highlights the organized contractile architecture of skeletal muscle and is consistent with the established localization of Alpha Actinin 2 (ACTN2), a major Z-disc-associated protein that cross-links actin filaments and supports sarcomere organization. This image illustrates the highly ordered structure of the muscle contractile apparatus and supports studies of muscle biology, sarcomere assembly, and cardiovascular research. The inset image represents a negative control in which PBS was used in place of the primary antibody and shows absence of specific staining. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.