NSJ Bioreagents

Propionyl-CoA carboxylase is a 2 subunit mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA.

PCCA antibody plays a crucial role in studying protein complex assembly and cellular processes in numerous research disciplines. Antibodies to Propionyl-CoA carboxylase alpha can be used to explore its applications in Western blot, immunohistochemistry, and immunofluorescence.

Western blotting is a widely used technique in molecular biology research to detect specific proteins in a sample. With the help of a PCCA antibody, researchers can effectively detect the presence of Propionyl-CoA carboxylase alpha protein in complex protein mixtures, providing valuable insights into protein expression levels and post-translational modifications.

Immunohistochemistry is a powerful technique used to visualize the distribution and localization of proteins within tissues. By utilizing a Propionyl-CoA carboxylase alpha antibody in immunohistochemical studies, researchers can precisely target and visualize the Propionyl-CoA carboxylase alpha protein within tissue samples, aiding in the identification of potential biomarkers and disease mechanisms.

Immunofluorescence is a fluorescence-based technique that allows for the visualization of proteins within cells with high spatial resolution. By employing a PCCA antibody in immunofluorescence studies, researchers can track the localization and dynamics of Propionyl-CoA carboxylase alpha protein within live cells, offering valuable insights into cellular processes and interactions.

A PCCA antibody is a valuable tool for researchers in the fields of molecular biology, cell biology, and immunology.

Researchers studying mitochondrial metabolism, amino acid catabolism, and cellular energy homeostasis pathways may also be interested in our Metabolism Antibodies landing page featuring metabolic enzymes, transport proteins, and energy regulation related targets.

Browse the complete collection of research antibodies on our Antibodies landing page.

<p><strong data-start="0" data-end="35" data-is-only-node="">PCCA Antibody Liver Cancer IHC.</strong> Immunohistochemistry analysis of FFPE human liver cancer tissue using PCCA Antibody demonstrates widespread HRP-DAB brown cytoplasmic staining throughout tumor cell populations. The staining pattern is consistent with the established mitochondrial localization of Propionyl-CoA carboxylase subunit alpha (PCCA), a key metabolic enzyme involved in branched-chain amino acid catabolism, propionate metabolism, and cellular energy homeostasis. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.</p>

PCCA Antibody Liver Cancer IHC. Immunohistochemistry analysis of FFPE human liver cancer tissue using PCCA Antibody demonstrates widespread HRP-DAB brown cytoplasmic staining throughout tumor cell populations. The staining pattern is consistent with the established mitochondrial localization of Propionyl-CoA carboxylase subunit alpha (PCCA), a key metabolic enzyme involved in branched-chain amino acid catabolism, propionate metabolism, and cellular energy homeostasis. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.