NSJ Bioreagents

AQP3 (Aquaporin 3) is a crucial protein that plays a significant role in the movement of water across cell membranes. Understanding its function and regulation is vital for various biological processes. Techniques such as western blot, immunohistochemistry, immunofluorescence, and flow cytometry use the AQP3 antibody to study AQP3.

Western blot analysis is a commonly used technique to detect and quantify proteins in a sample. By using an AQP3 antibody specific to the protein, researchers can identify the presence of AQP3 in different cell types or tissues. This technique provides valuable information about the expression levels of AQP3 under various conditions and can help unravel its physiological significance.

Immunohistochemistry and immunofluorescence are powerful tools that allow researchers to visualize the localization of AQP3 within cells or tissues. By using an AQP3 antibody tagged with a fluorophore, scientists can track the distribution of AQP3 in different cellular compartments. This information is essential for understanding the role of AQP3 in water transport and homeostasis.

Flow cytometry is another innovative technique that enables the quantification of AQP3 in individual cells. By using a fluorescently labeled AQP3 antibody, researchers can analyze the expression levels of AQP3 in a cell population and study its dynamic changes in response to stimuli. This high-throughput approach provides valuable insights into the regulation of AQP3 and its impact on cellular functions.