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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2361 to 2370
    PKC delta/theta Antibody / PRKCD/PRKCQ (clone 31P77)
    Catalog No : FY12804
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Mouse heart tissue lysate tested with PKC delta/theta Antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight: 68-82 kDa.
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    RPS8 Antibody / Ribosomal protein S8 (clone 30R72)
    Catalog No : FY12810
    Applications : WB, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of RPS8 using anti-RPS8 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS8 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band is observed at ~26 kDa, consistent with post-translationally modified RPS8 running slightly above its predicted ~24 kDa.
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    NDUFAB1 Antibody / NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1 (clone 30N94)
    Catalog No : FY12814
    Applications : WB, IHC, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of NDUFAB1 using anti-NDUFAB1 antibody. NDUFAB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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    CTNND2 Antibody / Delta 2 Catenin (clone 31C89)
    Catalog No : FY12815
    Applications : WB, ICC, IF
    Reactivity : Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Delta 2 Catenin expression in rat brain cell lysate using CTNND2 antibody. Predicted molecular weight ~133 kDa.
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    TNS4 Antibody / Tensin 4 (clone 30T19)
    Catalog No : FY12820
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of TNS4 expression in human HepG2 cell lysate using TNS4 antibody. A predominant band is observed at ~90 kDa, consistent with full-length TNS4/Cten migrating above its predicted ~78-80 kDa due to post-translational modification and gel mobility effects; lower fragments, when present, likely reflect caspase-dependent cleavage.
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    FETUB Antibody / Fetuin B (clone 31F67)
    Catalog No : FY12821
    Applications : WB, IHC
    Reactivity : Human, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Fetuin B expression in human serum cell lysate using FETUB antibody. A band just above 50 kDa is observed. The higher apparent size relative to the predicted ~42 kDa is consistent with glycosylated, secreted Fetuin-B.
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    Phospho-Phospholamban (pS16) Antibody / PLN (clone 32P55)
    Catalog No : FY12822
    Applications : WB
    Reactivity : Human, Mouse
    Format : Liquid
    Recrabbitmono
    Rat kidney tissue lysate tested with the Phospho-Phospholamban (pS16) antibody at 1:2000 dilution for 1 hour at room temperature. A specific band is detected near ~10 kDa, consistent with the phosphorylated form of monomeric Phospholamban. The apparent size exceeds its 6 kDa theoretical mass due to its hydrophobic, multimeric nature and phosphorylation-dependent gel mobility.
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    PEX11B Antibody / Peroxisomal membrane protein 11B (clone 29P66)
    Catalog No : FY12824
    Applications : WB, IHC
    Reactivity : Human, Mouse
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of PEX11B using anti-PEX11B antibody. PEX11B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PEX11B antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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    Phospho-CaMKII alpha (pT286) Antibody / CAMK2A (clone 31C26)
    Catalog No : FY12828
    Applications : WB, IHC
    Reactivity : Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Phospho-CaMKII alpha (pThr286) western blot of mouse brain using Phospho-CaMKII alpha (pT286) antibody. Lane 1, strong ~50/55 kDa doublet consistent with different phosphorylation states of CaMKII alpha; lane 2, lambda phosphatase treatment abolishes the signal, confirming phospho-specificity.
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    CrkL Antibody / Crk-like protein (clone 32C63)
    Catalog No : FY12832
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of CRKL using anti-CRKL antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for CRKL at approximately 37 kDa. The expected molecular weight of CRKL is 34-39 kDa.
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