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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2331 to 2340
    DUT Antibody / Deoxyuridine triphosphatase (clone 31D50)
    Catalog No : FY12685
    Applications : WB, ICC, IF, FACS
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of DUT expression in Ramos cell lysate using DUT antibody. Bands corresponding to the mitochondrial (~24-27 kDa) and the shorter nuclear/cytosolic (~18-21 kDa) isoforms can be detected; the ~18 kDa isoform can appear as a doublet consistent with modification- and processing-dependent mobility.
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    ATE1 Antibody / Arginyl transferase 1 (clone 30A62)
    Catalog No : FY12687
    Applications : WB, IHC
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of ATE1 expression in (1) human HepG2 cell lysate; (2) mouse spleen lysate. Predicted molecular weight ~59 kDa.
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    ELOB Antibody / Elongin B (clone 30E89)
    Catalog No : FY12689
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of TCEB2/ELOB expression in Jurkat cell lysate using ELOB antibody. Western blot shows a band at ~16 kDa, higher than the calculated ~13 kDa, consistent with the known slower SDS-PAGE migration of small ubiquitin-like proteins such as Elongin B.
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    AKT1/2/3 Antibody (clone 31A24)
    Catalog No : FY12694
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    All lanes use the AKT1/2/3 antibody at 1:5000 dilution for 1 hour at room temperature. Western blot analysis of AKT1/2/3 using a pan-AKT antibody. A principal band is detected at ~56 kDa with a faint higher-migrating band consistent with phosphorylated AKT and a very light lower species consistent with a less-phosphorylated or partially processed form.
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    LAG-3 Antibody / Lymphocyte activation gene 3 protein (clone 31L58)
    Catalog No : FY12697
    Applications : IF, IHC, ICC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Immunofluorescent analysis using the LAG-3 antibody (green) at 1:150 dilution.
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    HIF1A Antibody / HIF-1 alpha (clone 31H88)
    Catalog No : FY12702
    Applications : WB, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of HIF-1-Alpha using anti-HIF1A antibody. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Predicted molecular weight ~110 kDa, commonly observed at 100-120 kDa.
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    Histone H2B (acetyl K16) Antibody / HIST2H2BE (clone 31H96)
    Catalog No : FY12703
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse
    Format : Liquid
    Recrabbitmono
    Rat heart tissue lysate tested with the Histone H2B (acetyl K16) antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight ~15 kDa.
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    IL5RA Antibody / CD125 (clone 31I14)
    Catalog No : FY12710
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of CD125/IL5RA in human serum cell lysate using IL5RA antibody. Predicted molecular weight ~47 kDa but may be observed at higher molecular weights due to glycosylation.
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    INMT Antibody / Indolethylamine N-methyltransferase / TEMT (clone 29I89)
    Catalog No : FY12715
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of INMT/TEMT expression in human A549 cell lysate using INMT antibody. Predicted molecular weight ~29 kDa.
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    Alpha Tubulin (acetyl K40) Antibody / TUBA1B/TUBA4A (clone 31T79)
    Catalog No : FY12718
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    All lanes use the antibody at 1:2000 dilution for 1 hour at room temperature. Predicted molecular weight ~50 kDa.
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