DUT Antibody / Deoxyuridine triphosphatase [clone 31D50] (FY12685)

Western blot analysis of DUT expression in Ramos cell lysate using DUT antibody. Bands corresponding to the mitochondrial (~24-27 kDa) and the shorter nuclear/cytosolic (~18-21 kDa) isoforms can be detected; the ~18 kDa isoform can appear as a doublet consistent with modification- and processing-dependent mobility.

DUT Antibody Human Cell Line WB. Western blot analysis of HeLa, 293, MCF7, K562, and U-87 MG cell lysates using Deoxyuridine Triphosphatase / DUT Antibody demonstrates detection of DUT isoforms within the expected molecular weight range. Deoxyuridine Triphosphatase (DUT) is a nucleotide metabolism enzyme that regulates intracellular dUTP pools and protects genomic DNA from inappropriate uracil incorporation during replication. The predominant signal is observed at approximately 18-21 kDa, consistent with the mature nuclear/cytosolic DUT isoform, while higher molecular weight species corresponding to the mitochondrial precursor isoform may also be detected. The lower molecular weight isoform can appear as a doublet, reflecting post-translational modification and processing events associated with DUT maturation. Western blot was performed using a 1:5000 dilution of primary antibody. Predicted molecular weights: ~24-27 kDa (mitochondrial isoform) and ~18-21 kDa (nuclear/cytosolic isoform).

DUT Antibody HeLa IF. Immunofluorescent staining of HeLa cells using Deoxyuridine Triphosphatase / DUT Antibody demonstrates prominent nuclear localization of DUT (green), consistent with the expected distribution of the mature nuclear isoform responsible for regulating intracellular dUTP pools during DNA replication. DUT is a genome integrity enzyme that prevents inappropriate uracil incorporation into DNA and supports replication fidelity through maintenance of nucleotide homeostasis. Phalloidin-TRITC staining (red) highlights the actin cytoskeleton, while DAPI (blue) identifies cell nuclei. The observed nuclear staining pattern is consistent with the established role of DUT in nucleotide metabolism, DNA integrity, and genome maintenance pathways. Immunofluorescent staining was performed using DUT antibody at a 1:50 dilution.

Deoxyuridine Triphosphatase Antibody HeLa IF. Immunofluorescent staining of HeLa cells using Deoxyuridine Triphosphatase Antibody demonstrates strong nuclear localization of DUT (green), consistent with the expected distribution of the mature nuclear dUTPase isoform involved in regulation of nucleotide pools and preservation of DNA integrity. Deoxyuridine Triphosphatase is a nucleotide metabolism enzyme that prevents inappropriate uracil incorporation into DNA and supports genome maintenance during cellular proliferation. Phalloidin-TRITC staining (red) visualizes the actin cytoskeleton, while DAPI (blue) identifies cell nuclei. The observed staining pattern is consistent with the established role of DUT in DNA replication fidelity, nucleotide homeostasis, and genomic stability pathways. Immunofluorescent staining was performed using DUT antibody at a 1:150 dilution.