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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 1871 to 1880
    p16INK4a Antibody / CDKN2A (clone CDKN2A/8196R)
    Catalog No : V5281
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry of P16INK4a antibody in human bladder carcinoma tissue. The recombinant rabbit mAb clone CDKN2A/8196R demonstrates strong nuclear and cytoplasmic HRP-DAB brown staining within malignant urothelial cells, consistent with overexpression of Cyclin-dependent kinase inhibitor 2A in dysregulated cell cycle states. Tumor cell nests show diffuse positivity, while surrounding stromal elements display minimal background staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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    PD-L1 Antibody / B7-H1 / CD274 (clone PDL1/8408R)
    Catalog No : V5286
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human placental tissue with PD-L1 antibody (clone PDL1/8408R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    PD-L1 Antibody / B7-H1 / CD274 (clone PDL1/7568R)
    Catalog No : V5287
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of PD-L1 antibody in human lung squamous cell carcinoma. Programmed cell death 1 ligand 1 Antibody Rabbit MAb PDL1/7568R was used for immunohistochemistry on FFPE human lung squamous cell carcinoma tissue. Distinct HRP-DAB brown membranous staining is observed in tumor epithelial cells, consistent with the cell surface localization of Programmed death-ligand 1 (PD-L1 / CD274), an immune checkpoint ligand that regulates T cell responses within the tumor microenvironment. The staining highlights clusters of malignant squamous epithelial cells showing membranous PD-L1 expression, while surrounding stromal elements display minimal signal. The rabbit monoclonal antibody clone PDL1/7568R detects PD-L1 expression in tumor cells of lung squamous cell carcinoma following heat-induced epitope retrieval using pH 9 Tris-EDTA buffer.
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    MART-1 / Melan-A Antibody (clone MLANA/8108R)
    Catalog No : V5288
    Applications : IHC-P, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human COLO-38 cell lysate with recombinant MART-1 antibody (clone MLANA/8108R). Expected molecular weight ~20 kDa with possible doublet.
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    MART-1 / Melan-A Antibody (clone MLANA/8365R)
    Catalog No : V5290
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human melanoma tissue with Melan-A antibody (clone MLANA/8365R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    PD-L1 Antibody / B7-H1 / CD274 (clone PDL1/8222R)
    Catalog No : V5291
    Applications : WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human HDLM-2 cell lysate with PD-L1 antibody (clone PDL1/8222R). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
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    PD-L1 Antibody / B7-H1 / CD274 (clone PDL1/8591R)
    Catalog No : V5292
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of PD-L1 antibody in human placenta. PD-L1 Antibody Rabbit Monoclonal PDL1/8591R was used for immunohistochemistry on FFPE human placental tissue. Distinct HRP-DAB brown membranous staining is observed along the trophoblastic epithelial layer of chorionic villi, consistent with the known surface localization of Programmed death-ligand 1 (PD-L1 / CD274), an immune checkpoint ligand that contributes to regulation of maternal-fetal immune tolerance. The staining outlines the syncytiotrophoblast and cytotrophoblast layers surrounding villous structures, while underlying stromal components show minimal signal. The rabbit monoclonal antibody clone PDL1/8591R detects PD-L1 expression in placental trophoblastic epithelium within the villous architecture. Inset: PBS used in place of primary antibody as a secondary antibody negative control. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    PD-L1 Antibody / B7-H1 / CD274 (clone PDL1/8809R)
    Catalog No : V5293
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of B7-H1 antibody in human placenta. B7-H1 Antibody Recombinant Rabbit MAb PDL1/8809R was used for immunohistochemistry on FFPE human placental tissue. HRP-DAB brown membranous staining is observed in trophoblastic epithelial cells lining chorionic villi, consistent with the known cell surface localization of Programmed death-ligand 1 (PD-L1 / CD274), an immune checkpoint ligand involved in regulation of maternal-fetal immune tolerance. The staining highlights the trophoblastic layer surrounding placental villous structures, while underlying stromal components show minimal signal. The recombinant rabbit monoclonal antibody (clone PDL1/8809R) detects PD-L1 expression in placental epithelial cells within the villous architecture. Inset: PBS used in place of primary antibody as a secondary antibody negative control. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    TLE1 Antibody / Transducin-like enhancer protein 1 (clone TLE1/8007R)
    Catalog No : V5294
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human prostate carcinoma tissue with Transducin-like enhancer protein 1 antibody (clone TLE1/8007R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    FOXP3 Antibody / Scurfin (clone FOXP3/7990R)
    Catalog No : V5295
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human tonsil tissue with FOXP3 antibody (clone FOXP3/7990R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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