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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 1371 to 1380
    BAG1 Antibody / BAG-1L p50 specific (clone RM310)
    Catalog No : R20344
    Applications : WB, IHC-P, ICC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human HeLa cell lysate with recombinant BAG-1 antibody at 1:1000. Expected molecular weight of the BAG-1L isoform: ~50 kDa.
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    PD-L1 Antibody / B7-H1 / CD274 (clone RM320)
    Catalog No : R20345
    Applications : WB, IHC-P, ICC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human tumor cell line lysate with recombinant PD-L1 antibody at 1:1000. DU145 and LNCaP are prostate cancer cell lines with very minor PD-L1 expression. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
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    H3K9me2/K14acAntibody / Dimethyl-Histone H3 (Lys9) + Acetyl-Histone H3 (Lys14) (clone RM322)
    Catalog No : R20346
    Applications : WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    H3K9me2/K14ac Antibody / HIST1H3A Chromatin State Transition Antibody (clone RM322) specificity analysis. Peptide binding assay demonstrating selective recognition of HIST1H3A / Histone H3 only when both dimethyl-Lys9 and acetyl-Lys14 (K9me2/K14ac) modifications are present simultaneously. Strong signal is observed exclusively with the dual-modified peptide, while no detectable reactivity is seen with unmodified peptides, Lys9 methylation alone (K9me1, K9me2, K9me3), Lys14 acetylation alone, or other single-modification states, confirming high specificity for the combinatorial K9me2/K14ac chromatin state associated with dynamic epigenetic regulation.
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    PSA Antibody / Prostate Specific Antigen (clone RM323)
    Catalog No : R20347
    Applications : WB, IHC-P, ELISA
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Prostate Specific Antigen Antibody for WB western blot analysis of PSA and PSA-ACT complex. Western blot testing of purified PSA (from human seminal fluid), ACT (alpha 1-antichymotrypsin from human plasma), and the PSA-ACT complex under non-reducing (-) and reducing (+) conditions using Prostate Specific Antigen Antibody for WB (clone RM323) at 1:2500 shows a strong band corresponding to PSA at approximately 30-34 kDa. Under non-reducing conditions, a higher molecular weight band consistent with the PSA-ACT complex is observed near approximately 90 kDa, while the ACT control alone does not produce a detectable PSA band. Reduction disrupts the complex and results in detection of the PSA protein at its expected molecular weight. Ponceau S staining of the membrane is shown at left to confirm protein loading prior to immunoblot detection.
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    CALB2 Antibody / Calbindin 2 / Calretinin (clone RM324)
    Catalog No : R20349
    Applications : WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human A431 cell lysate with recombinant Calretinin antibody at 1:2000. Predicted molecular weight ~29 kDa.
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    Caveolin-1 Antibody (clone RM325)
    Catalog No : R20350
    Applications : WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human liver tissue with recombinant Caveolin-1 antibody at 1:1000.
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    CEA Antibody / Carcinoembryonic Antigen (clone RM326)
    Catalog No : R20351
    Applications : WB, IHC-P, ELISA
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human MCF7 cell lysate with recombinant CEA antibody at 1:000. Expected molecular weight: 80~200 kDa depending on glycosylation level.
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    FOLH1 Antibody / Folate Hydrolase 1/ PSMA (clone RM327)
    Catalog No : R20352
    Applications : WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human LNCaP cell lysate with recombinant PSMA antibody at 1:1000. Predicted molecular weight ~100 kDa.
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    Cytokeratin 14 Antibody / CK14 / KRT14 (clone RM328)
    Catalog No : R20353
    Applications : WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of human A431 cell lysate with recombinant Cytokeratin 14 antibody at 1:1000. Predicted molecular weight ~53 kDa.
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    HCG-beta Antibody / Choriogonadotropin subunit beta (clone RM330)
    Catalog No : R20354
    Applications : WB, IHC-P, ELISA
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of hCG protein purified from human urine using recombinant HCGb antibody at 1:20,000. Predicted molecular weight ~18 kDa (beta subunit) and ~26 kDa (hCG complex), but can be observed at up to ~40 kDa due to glycosylation.
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