BrdU Antibody [clone 85-2C8] (V3112)

Description

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Application Notes

Optimal dilution of the BrdU antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 min at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.

Storage

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).