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Home >> Antibodies >> BrdU Antibody

BrdU Antibody [clone BRD469] (V2321)

  Catalog No Formulation Size Price (USD)  
Image V2321-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V2321-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V2321SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469).
IHC staining of mouse intestine tissue (20X) with BrdU antibody (BRD469).
IHC/IF testing of FFPE rat kidney with Alexa Fluor 488 conjugated BrdU antibody.
Species Reactivity All species
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name BRD469
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This BrdU antibody is available for research use only.
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Description

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.

Immunogen

Bromodeoxyuridine conjugated to KLH was used as the immunogen.

Storage

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Bromodeoxyuridine, BUdr, BrdU antibody

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