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Home >> Antibodies >> GFAP Antibody

GFAP Antibody [clone GA-5] (V2129)

  Catalog No Formulation Size Price (USD)  
Image V2129-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2129-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2129SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2129IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC staining of FFPE human cerebellum with GFAP antibody (clone GA-5).
Western blot testing of human brain lysate GFAP antibody (clone GA-5). Predicted molecular weight ~50 kDa.
Western blot testing of human brain lysate GFAP antibody (clone GA-5). Predicted molecular weight ~50 kDa.
Flow cytometry testing of permeabilized human T98G cells with GFAP antibody (clone GA-5); Red=isotype control, Blue= GFAP antibody.
SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GA-5) as confirmation of integrity and purity.
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name GA-5
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2670
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This GFAP antibody is available for research use only.
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Description

This antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GFAP antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

GFAP isolated from pig spinal cord was used as the immunogen.

Storage

Store the GFAP antibody at 2-8oC.

Alternate Names

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein, GFAP antibody

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