Glial Fibrillary Acidic Protein Antibody SPM248

	Catalog No	Formulation	Size	Price (USD)
	V9022-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V9022-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V9022SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V9022IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC: Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (clone SPM248).

Western blot testing of human T98G cell lysate with Glial Fibrillary Acidic Protein antibody (clone SPM248). Expected molecular weight 50~55 kDa.

Flow cytometry testing of permeabilized human T98G cells with Glial Fibrillary Acidic Protein antibody (clone SPM248); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.

SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone SPM248) as confirmation of integrity and purity.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	SPM248
Purity	Protein G affinity chromatography
UniProt	P14136
Localization	Cytoplasmic
Applications	Flow cytometry : 1-2ug/10^6 cells Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1) (2)
Limitations	This Glial Fibrillary Acidic Protein antibody is available for research use only.
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Description

Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

The optimal dilution of the Glial Fibrillary Acidic Protein antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.