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Home >> Antibodies >> GFAP Antibody

GFAP Antibody [clone GA-5] (V2129)

  Catalog No Formulation Size Price (USD)  
V2129-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2129-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2129SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2129IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human brain stained with GFAP antibody (GA-5). Note cytoplasmic staining.
Western blot testing of human samples using GFAP antibody (GA-5).
Species Reactivity Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name GA-5
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2670
Localization Cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This GFAP antibody is available for research use only.

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Description

This antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GFAP antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

GFAP isolated from pig spinal cord was used as the immunogen.

Storage

Store the GFAP antibody at 2-8°C.

Alternate Names

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein, GFAP antibody

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