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Home >> Antibodies >> GFAP Antibody

GFAP Antibody (R31508)

  Catalog No Formulation Size Price (USD)  
R31508 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 299
Western blot testing of GFAP antibody and Lane 1: rat brain; 2: mouse brain; 3: U87; 4: SHG-44 (human glioma line); 5: NEURO; 6: HeLa lysate. Predicted molecular weight: ~50kDa.
IHC-P: GFAP antibody testing of human brain cancer
IHC-P: GFAP antibody testing of mouse brain tissue
IHC-P: GFAP antibody testing of rat brain tissue
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 2670
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This GFAP antibody is available for research use only.

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Description

Glial fibrillary acidic protein is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cells cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. It is necessary for many critical roles in the CNS. It also plays a role in astrocyte-neuron interactions as well as cell-cell communication.

Application Notes

The stated application concentrations are suggested starting points. Titration of the GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 93-432) was used as the immunogen for this GFAP antibody.

Storage

After reconstitution, the GFAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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