Zebrafish YAP1 Antibody / Yes Associated Protein Antibody (RZ1446)

Zebrafish YAP1 Antibody WB. Western blot analysis of YAP1 expression using anti-YAP1 antibody. YAP1, also known as Yes Associated Protein, is a key effector of the Hippo signaling pathway involved in tissue growth and regeneration. Proteins from zebrafish head and embryo tissue lysates were separated under reducing conditions and transferred to nitrocellulose membranes. Lane 1: zebrafish head tissue lysate. Lane 2: zebrafish embryo tissue lysate. Membranes were incubated with YAP1 antibody (0.5 ug/ml) overnight at 4°C, followed by HRP-conjugated secondary antibody detection and ECL development. A distinct band was detected at approximately 75 kDa in both samples, demonstrating expression of this Yes Associated Protein in zebrafish tissues. The predicted molecular weight of YAP1 is approximately 48 kDa; the slower migration may reflect post-translational modifications or altered electrophoretic mobility that have been reported for YAP1 proteins.