ZAP70 Antibody for FACS / T-cell Signaling Flow Cytometry Antibody [clone ZAP70/2047] (V3946)

ZAP70 Antibody for FACS / T-cell Signaling Flow Cytometry Antibody. Flow cytometry analysis of intracellular ZAP70 expression in PFA-fixed human Jurkat T lymphocyte cells using clone ZAP70/2047. Cells were fixed and permeabilized to enable detection of the cytoplasmic tyrosine kinase Zeta-chain-associated protein kinase 70 (ZAP70), a key mediator of T-cell receptor signaling. The blue histogram shows specific intracellular staining of ZAP70-positive Jurkat cells, while the red histogram represents the isotype control, demonstrating a clear rightward shift consistent with T-cell signaling-associated ZAP70 expression.

IHC testing of FFPE human tonsil with ZAP70 antibody (clone ZAP70/2047). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human Jurkat cells with ZAP70 antibody (green, clone ZAP70/2047) and Reddot nuclear stain (red).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZAP70 antibody (clone ZAP70/2047). These results demonstrate the foremost specificity of the ZAP70/2047 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free ZAP70 antibody (clone ZAP70/2047) as confirmation of integrity and purity.