ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody [clone ZAP70/2035] (V3942)

ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody. Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tonsil tissue using ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody (clone ZAP70/2035). Antigen retrieval was performed by boiling sections in pH 9 Tris-EDTA buffer for 10-20 minutes followed by cooling at room temperature. Cytoplasmic HRP-DAB brown staining is observed in lymphoid cell populations, with stronger staining in interfollicular regions surrounding lighter germinal centers. This pattern is consistent with Zeta-chain-associated protein kinase 70 (ZAP70) expression in cytotoxic T lymphocytes, highlighting effector cell-rich areas and supporting its role in cytotoxic lymphocyte signaling within tonsillar tissue architecture.

ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody. Immunofluorescence analysis of PFA-fixed human Jurkat T lymphocyte cells using ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody (clone ZAP70/2035). ZAP70 antibody staining (green) shows predominantly cytoplasmic localization consistent with Zeta-chain-associated protein kinase 70 (ZAP70) function in cytotoxic lymphocyte signaling pathways, while Reddot nuclear stain (red) marks cell nuclei. The staining pattern supports visualization of intracellular signaling components associated with cytotoxic T-cell function and highlights ZAP70 distribution within lymphocyte populations by immunofluorescence imaging.

ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody. Flow cytometry analysis of PFA-fixed human Jurkat T lymphocyte cells using ZAP70 Antibody / Cytotoxic Lymphocyte Signaling Antibody (clone ZAP70/2035). Cells were fixed and permeabilized to enable intracellular detection of Zeta-chain-associated protein kinase 70 (ZAP70), a cytoplasmic tyrosine kinase involved in cytotoxic lymphocyte signaling pathways. The blue histogram shows a clear rightward fluorescence shift compared to the red isotype control, indicating specific intracellular ZAP70 staining. This pattern supports detection of signaling-associated ZAP70 expression in lymphocyte populations relevant to cytotoxic immune responses.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZAP70 antibody (clone ZAP70/2035). These results demonstrate the foremost specificity of the ZAP70/2035 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free ZAP70 antibody (clone ZAP70/2035) as confirmation of integrity and purity.