YWHAG Antibody | 14-3-3 Gamma Isoform Antibody

	Catalog No	Formulation	Size	Price (USD)
	RQ7260	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

YWHAG Antibody Human Esophageal Squamous Carcinoma IHC. IHC staining of FFPE human esophageal squamous carcinoma tissue using YWHAG Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling prior to staining. Positive YWHAG staining is observed in the carcinoma cells. This YWHAG Antibody, also called a 14-3-3 Gamma Isoform Antibody, demonstrates YWHAG expression in human esophageal squamous carcinoma and supports studies of phosphoprotein interactions and phosphorylation-dependent signal transduction.

YWHAG Antibody Human, Mouse and Rat WB. Western blot analysis of YWHAG in human Jurkat cell and placenta tissue lysates, rat brain and skeletal muscle tissue lysates, and mouse brain and skeletal muscle tissue lysates using YWHAG Antibody. Lane 1: Jurkat; Lane 2: human placenta; Lane 3: rat brain; Lane 4: rat skeletal muscle; Lane 5: mouse brain; Lane 6: mouse skeletal muscle. A specific band is detected at approximately 28 kDa, consistent with the predicted molecular weight of YWHAG. This YWHAG Antibody, also called a 14-3-3 Gamma Isoform Antibody, detects YWHAG across human, rat and mouse samples and supports studies of phosphoprotein interactions and phosphorylation-dependent signal transduction.

YWHAG Antibody U-87 MG Cell FACS. Flow cytometry analysis of human U-87 MG cells using YWHAG Antibody at 1 ug/million cells. Cells were blocked with goat serum prior to staining. The histogram shows YWHAG staining (blue), isotype control (green) and unstained cells (red). A positive fluorescence shift is observed with YWHAG staining relative to the isotype control. This YWHAG Antibody, also called a 14-3-3 Gamma Isoform Antibody, detects YWHAG in human U-87 MG cells and supports studies of phosphoprotein interactions and phosphorylation-dependent signal transduction.

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YWHAG Antibody Human Esophageal Squamous Carcinoma IHC. IHC staining of FFPE human esophageal squamous carcinoma tissue using YWHAG Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling prior to staining. Positive YWHAG staining is observed in the carcinoma cells. This YWHAG Antibody, also called a 14-3-3 Gamma Isoform Antibody, demonstrates YWHAG expression in human esophageal squamous carcinoma and supports studies of phosphoprotein interactions and phosphorylation-dependent signal transduction.

YWHAG Antibody Human, Mouse and Rat WB. Western blot analysis of YWHAG in human Jurkat cell and placenta tissue lysates, rat brain and skeletal muscle tissue lysates, and mouse brain and skeletal muscle tissue lysates using YWHAG Antibody. Lane 1: Jurkat; Lane 2: human placenta; Lane 3: rat brain; Lane 4: rat skeletal muscle; Lane 5: mouse brain; Lane 6: mouse skeletal muscle. A specific band is detected at approximately 28 kDa, consistent with the predicted molecular weight of YWHAG. This YWHAG Antibody, also called a 14-3-3 Gamma Isoform Antibody, detects YWHAG across human, rat and mouse samples and supports studies of phosphoprotein interactions and phosphorylation-dependent signal transduction.

YWHAG Antibody U-87 MG Cell FACS. Flow cytometry analysis of human U-87 MG cells using YWHAG Antibody at 1 ug/million cells. Cells were blocked with goat serum prior to staining. The histogram shows YWHAG staining (blue), isotype control (green) and unstained cells (red). A positive fluorescence shift is observed with YWHAG staining relative to the isotype control. This YWHAG Antibody, also called a 14-3-3 Gamma Isoform Antibody, detects YWHAG in human U-87 MG cells and supports studies of phosphoprotein interactions and phosphorylation-dependent signal transduction.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P61981
Localization	Cytoplasmic
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This YWHAG Antibody / 14-3-3 Gamma Isoform Antibody is available for research use only.
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Description

YWHAG Antibody / 14-3-3 Gamma Isoform Antibody recognizes YWHAG, the gene product encoding the gamma isoform of the 14-3-3 family of regulatory proteins. 14-3-3 proteins function primarily through interactions with other cellular proteins, frequently recognizing phosphoserine- or phosphothreonine-containing motifs generated by kinase signaling. Binding to 14-3-3 gamma can alter the localization, stability, conformation or molecular interactions of its partners. Through these phosphorylation-dependent associations, YWHAG participates in intracellular signaling networks controlling cell survival, proliferation, apoptosis and other cellular responses.

The 14-3-3 family consists of several highly conserved isoforms that can form homo- and heterodimers. These dimers provide interaction surfaces capable of binding numerous signaling proteins and coordinating their behavior following phosphorylation. Rather than functioning as an enzyme, 14-3-3 gamma acts primarily as a regulatory adaptor. Association with YWHAG can sequester proteins within particular cellular compartments, protect phosphorylated residues from phosphatases, influence protein stability or facilitate formation of multiprotein signaling complexes. A YWHAG Antibody can therefore support studies examining how phosphorylation events are translated into downstream changes in protein function.

14-3-3 gamma has been linked to signaling mechanisms involved in cell-cycle progression, proliferation and apoptosis. Members of the 14-3-3 family interact with numerous components of kinase cascades and cell-survival pathways, providing an interface between phosphorylation-dependent signaling and downstream cellular responses. Regulation of protein localization is particularly important to 14-3-3 biology because binding can influence whether signaling and transcriptional regulatory proteins remain in the cytoplasm or gain access to other cellular compartments.

YWHAG is broadly expressed and has been studied extensively in neuronal and developmental biology as well as cancer research. The gamma isoform is expressed in the nervous system, where phosphorylation-dependent protein interactions contribute to neuronal development, signaling and cellular function. Altered YWHAG expression or regulation has also been investigated in tumors and other conditions involving disrupted signaling networks. Because the different 14-3-3 isoforms have substantial sequence similarity and overlapping interaction partners, distinguishing the gamma isoform can be important when investigating isoform-specific expression and regulation.

The ability of YWHAG to interact with numerous phosphorylated proteins places 14-3-3 gamma within a broad network connecting kinase activity with downstream cellular regulation. Its functions as a phosphoprotein-binding adaptor complement the activities of kinases, phosphatases and other signaling proteins that determine cellular responses to internal and external signals. NSJ Bioreagents supplies antibodies to YWHAG and related proteins involved in intracellular signaling. A YWHAG Antibody can support research on the 14-3-3 gamma isoform, phosphoprotein interactions, cell survival and phosphorylation-dependent signal transduction.

For additional information on the gamma member of the 14-3-3 protein family and its roles in phosphorylation-dependent signaling and intracellular regulation, see our 14-3-3 Gamma Antibody page.

Application Notes

Optimal dilution of the YWHAG Antibody / 14-3-3 Gamma Isoform Antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D21-K162) was used as the immunogen for the YWHAG antibody.

Storage

After reconstitution, the YWHAG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

YWHAG antibody, 14-3-3 Gamma Isoform antibody, 14-3-3 Gamma antibody, 14-3-3 Protein Gamma antibody, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma antibody

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YWHAG Antibody / 14-3-3 Gamma Isoform Antibody (RQ7260)

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NSJ Bioreagents